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OBJECTIVE@#To investigate the effect of p-coumaric acid on apoptosis of multiple myeloma cells and its related mechanism.@*METHODS@#Multiple myeloma cell line MM.1s cells were selected and treated with different concentrations of p-coumaric acid (0, 0.4, 0.8, 1.6, 3.2 mmol/L), and the inhibition rate and half inhibition concentration (IC50) were detected by CCK-8 method. Then MM.1s cells were treated with 1/2 IC50, IC50, 2 IC50 and transfected with ov-Nrf-2 and ov-Nrf-2+IC50. The apoptosis, ROS fluorescence intensity and mitochondrial membrane potential of MM.1s cells were detected by flow cytometry, and the relative expressions of cellular Nrf-2 and HO-1 protein were detected by Western blot.@*RESULTS@#P-coumaric acid inhibited the proliferation of MM.1s cells in a dose-dependent manner(r =0.997) with an IC50 value of 2.754 mmol/L. Compared with the control group, apoptosis and ROS fluorescence intensity of MM.1s cells were significantly increased in the 1/2 IC50 group, IC50 group, 2 IC50 group and ov-Nrf-2+IC50 group (P <0.01), the expressions of Nrf-2, HO-1 protein in the IC50 group and 2 IC50 group were significantly decreased (P <0.05). Compared with the IC50 group, the cells apoptosis and ROS fluorescence intensity were significantly decreased (P <0.01), and the expressions of Nrf-2 and HO-1 protein were significantly increased in the ov-Nrf-2+IC50 group (P <0.01).@*CONCLUSION@#P-coumaric acid can inhibit the proliferation of MM.1s cells and may target the Nrf-2/HO-1 signaling pathway to affect oxidative stress in MM cells thereby inducing their apoptosis.
Subject(s)
Humans , Reactive Oxygen Species/pharmacology , Cell Line, Tumor , Multiple Myeloma , Oxidative Stress , ApoptosisABSTRACT
Objective: To study the therapeutic effect and mechanism of alcohol extract from Epimedii Herba (AEEH) on ovariectomy-induced osteoporosis in rats. Methods: Ipriflavone Tablets (200 mg/kg) and different doses of AEEH (170 and 85 mg/kg) were given for 8 weeks by ig administration, from 4 weeks after ovariectomization. Then, the protein expression of serum ALP, StrACP, femoral osteoprotegerin (OPG), and osteoclast differentiation factor (RANKL) was evaluated by biochemical assay kits and immunohistochemical evaluation; The mechanical properties of the tibia were determined by three point bending test method. Finally, the distal femoral trabecular bone parameters and the 3D reconstruction, trabecular bone of the 3D graphics were intuitively illustrated, and the effect of AEEH on rats suffered from osteoporosis role was observed by micro-CT. Results: Treatment with AEEH (170 mg/kg) significantly inhibited serum ALP and StrACP levels. Furthermore, AEEH also could significantly increase OPG protein expression while reduce RANKL protein expression compared with OVX rats. In addition, it could effectively improve the biomechanical properties of the tibia and the parameters of the trabecular bone of the femur, respectively. Conclusion: AEEH is effective in treating ovariectomy-induced osteoporosis in rats and the mechanism is mainly achieved by promoting the activity of osteoblast and inhibiting the activity of osteoclast.
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Objective: To assess the potential effect of the low glycosides fraction from Epimedii Herba (LGFEH) on UMR-106 cells and zebrafish model with osteoporosis induced by Prednisolone. Methods: The MTT assay was used to detect the effect of LGFEH on the proliferation of UMR-106 cells, and at the same time, the influence of LGFEH to the UMR-106 cells differentiation was observed through testing the ALP activity of UMR-106 cells using biochemical assays. Zebrafish larvae at 3 d post fertilization were divided into blank control group, 0.5% DMSO group, Prednisolone group, etidronate disodium group, and LGFEH (1, 2, 4, 8, and 16 μg/mL) groups. All groups were incubated in 24-well plates for 9 d until execution, and then zebrafish skeleton was anesthetized and fixed for staining with alizarin red. Quantitative analysis of the stained area was performed by microscopic inspection and digital imaging methods to reflect the amount of bone mineralization. Results: The LGFEH significantly increased both proliferation and ALP activities of UMR-106 cells. Furthermore, compared with model group, head skeleton mineral area and IOD of the LGFEH groups were significantly increased. Conclusion: Our results indicate that the LGFEH might be beneficial for treating osteoporosis.
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To observe the effect of Epimedii Herba alcohol extract (HE) on tumor growth of lung cancer by establishing the model of Lewis tumor-bearing mice, ELISA method was used to detect the levels of TNF-α, IL-10, IL-17, IL-2 in serum. Ki67 and P53 protein expression was detected in lung cancer tissues by using Western blot assay method and immunohistochemical assay method. The experimental results showed that HE has certain inhibitory effect on Lewis lung cancer tumor growth, and it can reduce the levels of TNF-α, IL-10 and IL-17 in serum, improve the level of IL-2,significantly decrease the expression of Ki67, and significantly increase P53 expression. HE has obvious inhibitory effect against lung cancer, and has the ability to improve immune regulating effect. This study reveals the anti-lung cancer effect of HE may be related to its ability of improving immunity, thus provides the basis for further research on anti-lung cancer effect of HE.
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To analyze and compare the chemical compositions of Moutan Cortex, Paeoniae Rubra Radix and Paeoniae Alba Radix based on "component structure" theory. Thirteen batches of Moutan Cortex, 14 batches of Paeoniae Rubra Radix from different origins and 10 batches of Paeoniae Alba Radix from different origins were analyzed by HPLC-DAD method. Hierarchical cluster analysis and principal component analysis were used for analysis. The significant differences of principal component from Moutan Cortex, Paeoniae Rubra Radix and Paeoniae Alba Radix were investigated by using F test. HPLC fingerprints were established for 13 batches of Moutan Cortex, 14 batches of Paeoniae Rubra Radix and 10 batches of Paeoniae Alba Radix, and 7 glycosides and phenolic acids components were identified. Comparative study of Moutan Coetex, Paeoniae Rubra Radix and Paeoniae Alba Radix was conducted according to the results of hierarchical cluster analysis, principal component analysis and "component structure" theory. Moutan Cortex, Paeoniae Rubra Radix and Paeoniae Alba Radix have significant differences in mass fraction of major chemical components and their ratios, leading to different curative effects.
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Alisma orientalis is a traditional herb medicine commonly used in clinical. With the increasing report of its toxicity in clinical, the renal toxicity of Alisma orientalis has got gradually attention. This paper systematically reviews the research on the chemical material basis of Alisma orientalis including its chemical composition and toxicity of ingredients; and also declares its toxic ingredients and targets according to Network toxicology. Based on the controversy on renal toxicity of Alisma orientalis, we analyzed the possible reasons that may be associated with renal toxicity. It might be associated with the differences of the material basis composition and regulatory toxicology network, differences in employed processing technology, the metabolic function leading to accumulation of compounds, dosage and duration of the experiment and compatibility. The review provides possible reference and ideas for the quality control and rational use of Alisma orientalis.
Subject(s)
Animals , Humans , Alisma , Chemistry , Toxicity , Drugs, Chinese Herbal , Chemistry , Toxicity , Molecular StructureABSTRACT
Development of the disease is the result of several factors involved in biological network changes. The nature of drug intervention is to regulate these pathological changes to the normal range. Advantages of traditional Chinese medicine (TCM) are to integrally and systematically regulate this biological networks and systematic pathology through multi-targets, multi-levels, multi-channels. Structural components TCM provides the controlled and precise basis "substance" for this regulation and also to clarify the "truth" of the nature of the regulation by the network pharmacology. Network pharmacology provides new strategy for the research on mechanism of structural components TCM. This study not only reflects the overall characteristics of the development of the disease, but also fully embodies the essence of TCM for preventing and treating diseases through changing traditional model on "one drug, one gene, one disease". This paper explores systematically the integration essence, features and research strategies of structural components TCM and the network pharmacology, understand the interaction of structural components TCM and body from the perspective of the overall concept of improving or restoring the balance of.biological networks. It is effective measure to reveal the structure of a multi-component for regulating biological networks mechanisms, and also provide new ideas and methods for further scientific research and innovation of structural component TCM.